Effect of dark-incubation and inhibitor treatments on size and distribution of charasomes stained by fluorescent FM1-43 in branchlet internodal cells of Chara australis. Under standard conditions of 14/10 h light/darkness, extended charasome-rich areas (A) alternate with small, charasome-poor or -free regions ( B, and smaller magnification C). The white arrow in (C) indicates the neutral line, while the arrow head points to an FM-stained epiphyte.

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Lost Horizon 1973 Rapidshare Files. Panels (D) and (E) show different magnifications of cells exposed to continuous darkness for 12 days (control for G–J). Charasomes are mostly small and uniformly distributed (D), but “islands” with larger charasomes occasionally remained (F).

(G–J) Effect of 1 μM ikarugamycin (IKA; G), 1.5 μM filipin (H), and of a combination of both inhibitors (I, J) on dark-treated cells. Note the extended charasome-rich regions (J). Bars are 10 μm (A,B,D,E,G–I) and 50 μm (C,F,J). Coated Vesicles Are Involved in Charasome Degradation These findings prompted us to inspect the fine structure of dark-exposed cells, in order to decipher the mechanism(s) of charasome degradation. Figure shows an electron micrograph of a branchlet internodal cell, exposed to standard light/dark conditions. The charasomes consist of a complex meshwork of anastomosing tubules formed by plasma membrane invaginations. CPs and CVs with an average inner diameter of 65.7 ± 11. Cccam Info Plugin Enigma2 Download. 0 nm ( n = 77) were occasionally seen at the smooth plasma membrane between charasomes, but rarely at the cytoplasmic surface of charasomes, indicating that these organelles were no longer growing and can thus be called “mature” (compare;; ).